The following have all affiliated for the 2008/09 season, list last updated 18/07/2008
- Altrincham Kersal
- Ashford
- Bath Ladies
- Bridgnorth WRFC
- Buckingham Swans
- Chesham
- Darlington Mowden Park Sharks
- Ealing Jades
- Eccleshall Ladies
- Fakenham Foxes
- Harlequin Ladies
- Harlow Ladies
- Hucclecote Ladies
- Marlow Ladies
- New Ash Green Ladies
- Northwich Ladies
- Oakmeadians
- Old Leamingtonians Ladies
- Ormskirk Ladies
- Peterborough Ladies
- Plymouth Marjon Community Club
- Romford & Gidea Park Ravens
- Saracens 1st XV
- Saracens 2nd XV
- Southwold Swallows
- St Marys Old Boys Ladies
- Supermarine Ladies
- TNT North Tawton
- Trojans FC Ladies
- Vagabonds Ladies
- Witney Angels
- Worthing
- York RI Ladies
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